For protection of diverse genetic resources of local landraces and to get the benefit for next generation, research works should be continuing through screening of local landraces by using with local germplasm; which will be very useful to conserve the genetic variability and will provide to economic profits to the farmers by improving their uses. In the context, One hundred twenty-five maize landraces with two commercial maize hybrids (‘Kalumet’ and ‘Katone’) were evaluated for yield and agro-morphological performance for genetic improvement of future maize varieties. The landraces were collected from the Black Sea Region of Turkey and were evaluated under the augmented complete design under Diyarbakir agro-ecological conditions during 2015. After observation, it was observed that all genotypes showed a significant variations for all traits especially for yield and yield attributes. Considering the overall performance of all landraces, the days to tasseling and silking were varied from 39.5 to 64.5 and from 49.5 to 70.5 days; while the SPAD meter were varied from 37.8 to 70 unit, the plant height from 165 to 315.5 cm, the ear height from 55.8 to 190 cm, the stalk thickness from 11.3-26 mm, the ear length from 6.21 to 25.38, the ear diameter 14.13 to 48.92 mm, the rows ear-1 from 2.33 to16.3, seed srow-1, the ear weight from 10.2 to 285.26g, the rachis diameter from 11.58 to 39.51mmand the grain yield from 63.68 to 1498.13 kg ha-1. Where, the range of landraces for all traits were varied huge and exceeded commercial check genotypes. Therefore, it was determined that the genotypic distinction of the landraces may be used as pre-breeding material for developing the suitable maize varieties for sustainable maize production in diverse agro-ecological conditions of Mediterranean region including Turkey.
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Two hundred and twenty eight (228) one day old quails were used to assess the suitability of cassava vinasse meal (CVM) as a replacement for maize at varying inclusion levels of 0.0%, 5.0%, 10.0% and 15.0%. The birds were fed an adequate starter diet for the first week before being randomly alloted into 4 dietary group of 3 replicate of 19 birds each. The feeding trial lasted for six weeks. The crude protein, crude fibre, ether extract, ash, moisture, nitrogen free extract and metabolizable energy of the dehydrated cassava vinasse were 19.26%, 7.96%, 3.72%, 9.33%, 5.68% and 12.17 MJ kg-1 ME respectively. The results showed that final weight, average daily weight gain and feed conversion ratio were significantly (P <0.05) influenced as inclusion level of cassava vinasse meal increases. Significant differences were observed on the haematological parameters such as haematocrit and mean corpuscular volume (P <0.05). The analyzed panelist response on organoleptic parameters showed that tenderness, juiciness and texture were significantly different (P <0.05) with birds fed 10.0% CVM having the least values. In conclusion, 10.0% CVM (21% replacement for maize) in the diet of quails had no deleterious effect on the feed conversion ratio, haematological parameters and meat acceptability. Moreover, further research could be geared towards the use of exogenous enzymes and the performance of other poultry species including broiler chicken.
The growth performance, economic indices, carcass cuts and organoleptic properties of pigs (large white × landrace, n = 30) fed biscuit dough as a replacement for maize was examined in a 49-day feeding trial. Weaned pigs were randomly allotted to five dietary groups of six pigs each. A maize-soybean meal based diet served as the control (D1) while diets D2, D3, D4, and D5 had 12.5%, 25.0%, 37.5% and 50.0% biscuit dough respectively as a replacement for maize. The feed conversion ratio (FCR), feed cost per kg, feed cost per kg weight gain, profit per kg weight and economic efficiency of gain (EEG) were significantly influenced (P <0.05) by the dietary treatments. The feed cost per kg and feed cost per kg weight gain reduced linearly from D1-D5, unlike the profit and EEG which increased linearly. The carcass primal cuts including jowl, boston butt, loin, spare rib, belly, ham, trotters, head and picnic shoulder were significantly different (P <0.05). The organoleptic properties (meat colour, texture and overall acceptability) were significantly influenced (P <0.05) by the dietary treatments. In conclusion, feeding up to 37.5% biscuit dough as a replacement for maize improved the feed conversion ratio. Nevertheless, the use of biscuit dough up to 50% would result in reduced cost of production, higher profit margin, economic efficiency of gain, greater loin, belly, ham and overall consumer acceptability. Therefore, the use of biscuit dough by pig farmers would improve pig performance and enhance profitability up to 50.0% replacement for maize.
This investigation tested the hypothesis that community structure of AMF would vary in soil sown with maize (Zea mays) and amended with inorganic NPK fertiliser and tillage practice. Three tillage practices [conventional (CT), minimum (MT) and zero (NT)] were imposed on maize cultivars (Oba super 2 and Swan 1) in soil amended with (600 kg NPK ha-1) and without. The treatments were in split-split arrangement fitted into randomised complete block design, replicated thrice. The main plot consisted of maize varieties; sub plot consisted of tillage practises, while sub-sub plot was made of soil amendments. The results showed NT supported enriched community structure of AMF with predominance of Glomus in soil in the order NT > MT > CT. Similar pattern was observed on species richness, species evenness and diversity. Spore count, percentage colonisation, species richness, evenness and diversity were significantly higher in non-amended soil. Significantly higher spore count was observed in the rhizosphere of Oba super 2. Improved performance of maize in amended soil could have implied complimentary role of Glomus apart from nutritional. Cultivar differences of maize and seasons could have explained variation in species diversity of AMF in a derived Savanna.
Arbuscular mycorrhizal fungi (AMF) are often negatively affected in agro-systems. This investigation tested the hypothesis that community structure of AMF would vary in soil sown with maize (Zea mays L.) and amended with inorganic NPK fertilizer and tillage practice; that varietal variation of maize would have implication on their performance in a derived savanna. Field experiments were conducted at the Teaching and Research Farm, Federal University of Agriculture, Abeokuta in the early and late cropping seasons of 2013. Three tillage practices [conventional (CT), minimum (MT) and zero (NT)] were imposed on maize cultivars (Oba super 2 and Swan 1) in soil amended with NPK fertilizer (120 kg N/ha + 60 kg P2 O5 /ha + 60 kg K2 O/ha) and no fertilization. The treatments were in split-split arrangement fitted into randomized complete block design, replicated thrice. The main plot consisted of tillage practises, the sub plot consisted of maize varieties, while the sub-sub plot was made of soil amendments. Glomus was identified in the soil in the order NT > MT > CT (early and late seasons of 2013). Similar pattern was observed on specie richness (late season), specie evenness and diversity (both seasons). Spore count, percentage AMF colonization, specie richness, evenness and diversity were significantly higher in non-amended soil than amended. Significantly higher spore count was observed in the rhizosphere of Oba super 2 than Swan 1. Conversely more Acaulospora was observed in Swan 1 than Oba super 2. These evidences suggested that NT supported enriched community structure of AMF with a predominance of Glomus. Conversely, amending soil with NPK in this agroecology reversed this pattern, except for Glomus. Improved performance of maize in amended soil could have implied complimentary role of Glomus apart from nutritional. Cultivar differences of maize and seasons could have explained variation in species diversity of AMF in a derived savanna.
We conducted a long term experiment (set up in 1983) to examine the effects of fertilization and plant density on SPAD and LAI values as well as yield in 2017 and 2018, in two maize hybrids (Sushi, Fornad) in chernozem soil. Hybrid yields varied between 10.2– 16.7 t ha-1 in 2017 and between 9.3–15.9 t ha-1 in 2018, depending on fertilization doses and plant density. The hybrids had SPADmax values in early July (55.5–60.4 in 2017 and 54.9–63.9 in 2018), whereas they got LAImax values in early July (3.0–5.3 m2 m-2 in 2017) and early August (3.5–4.4 m2 m-2 in 2018). Research data evaluated by Pearson correlation calculations proved that fertilization was the main factor that had a significant effect on SPAD and LAI values in the different maize phenophases (r = 0.6**–0.8** for SPAD, r = 0.5**–0.8** for LAI). Correlation among plant density, hybrid and SPAD and LAI values showed very weak correlations in both years (r = 0.1–0.3). The yield of the maize hybrids was most significantly affected by fertilizer in 2017 (r = 0.672**) and plant density in 2018 (r = 0.517**).
The present study deals with examining the response of maize to osmotic stress condition. In the experiment, two investigated plants which are maize were chosen to be tested in the controllable environment. One plant was controlled to be in well water condition, whereas another one was treated by PEG-6000 for being encountered the osmotic stress condition throughout the experimental period. The main measuring technique used to monitor the tested plants in the experiment consisted of acoustic emission techniques. The experimental results indicated that the distinct reactions between a control plant and an influenced plant according to osmotic stress condition could be differentiated by acoustic emission method.
The breeding studies targeted to develop high yielding varieties in maize have led to a decrease in genetic variation in secondary biochemical components. Local maize landraces are important genetic sources for these components. The objective of this study was to examine the genetic variation for phytic acid and total phenolic compounds within 192 Turkish maize landraces. The plant material was grown during the summer season of 2017 in Çanakkale, with the inclusion of 7 check hybrids. The field trial used an Augmented Experimental Design, with 6 blocks, each one containing 32 landraces and 7 check hybrids. Phytic acid and total phenolics were detected spectrophotometrically in the seeds of landraces propagated by bulk pollination. The data were subjected to analysis of variance and some genetic estimations (coefficients of variation, heritability, genetic advance) were calculated for the observed traits. Results of data analysis suggest that there is a considerable genetic variation among the investigated genetic materials. The phytic acid content was found between 0.82–4.87 mg g-1 and the total phenolic content was between 0.03–1.99%. For both traits, genetic variation in local maize landraces was observed to be wider than check varieties. The promising materials among landraces may have potential use in the future breeding programs for manipulating the levels of phytic acid and phenolic compounds. According to the calculations made for the inheritance of the traits, it was determined that the heritability in phytic acid content was higher (56.2%) than those for the total phenolics. Genetic gain calculations showed that genetic improvement can be achieved by selection for both investigated traits.
The study was undertaken at Regional Agricultural Research Station of Bangladesh Agricultural Research Institute, Ishurdi, Pabna, Bangladesh in consecutive two years of 2015 and 2016 for evaluating the performance of maize and Indian spinach intercropping (MIS) under different row spacing for higher profitability through economic assessment. Five different cropping systems viz., T1 : hybrid maize normal row along with one row of spinach, T2 : hybrid maize paired row along with one row of spinach, T3 : hybrid maize paired row along with two rows of spinach, T4 : hybrid maize paired row along with three rows of spinach and T5 : sole maize were included in this experiment. The performance of the component crops was evaluated regarding total grain and vegetable yield, competitive functions, and economic returns. The results indicate that yield and yield contributing characters of maize were not significantly affected by the intercrop of spinach at different ratios. Maize paired row along with three rows of spinach (IS) (T4 ) combination had higher maize equivalent yield (11.06 t ha-1), gross return (US$ 1,499 ha-1), gross margin (US$ 564 ha-1) and benefitcost ratio (BCR) (2.07) than others treatments. This intercropping system also recorded higher land equivalent ratio, monetary advantage index, land equivalent coefficient, income equivalent ratio and area time equivalent ratio of 1.24, US$ 2,434 ha-1, 0.30, 1.24 and 1.18, respectively. Hence, this cropping system may adopt in the smallholders’ for getting maximum benefits in prevailing climate change.
Genetically modified (GM) maize and traditional maize were tested experimentally to determine their effect on chicken serum biochemical parameters and metabolism. A total of 600 day-old chicks were divided into 2 experimental groups, namely: K, diet based on traditional maize (control); D, a diet based on GM maize (experiment). The experiment lasted 17 weeks, daily monitoring of population viability and feed consumption were carried out, at the age of 4, 8 and 17 weeks all birds were individually weighed. Blood samples for biochemical studies were taken from chickens at 4 weeks and 12 weeks of age (n= 6). Analytical studies did not show significant changes in serum biochemical parameters in group D chickens. A significant difference between the groups of chickens receiving GM and traditional maize was found only by the content of lipids after 4 weeks (P < 0.05) and cholesterol after 12 weeks of the experiment (P < 0.001). Also noted increasing level of CIC in serum of chickens that consumed feed from GM maize relative control at 32.2% (P < 0.01) after 4 weeks of the experiment. However, the studied indicators of metabolism and immune response in birds were within the physiological range. Certain fluctuations in the parameters during the experiment were observed in both groups, which indicates that there is no connection between this fact and the feeding factor. In addition, optimal results were obtained in terms of feed conversion and body weight of poultry, regardless of the feeding ration. The body weight of chickens at the age of 17 weeks in both groups was in the range of 1862.4-1895.6 g, feed consumption per 1 kg of body weight gain was 3.65-3.76 kg, the livestock viability – 96.6-97.3%. The results of the study do not indicate any danger to the metabolism and health of the bird due to the use of genetically modified feed, as no statistically significant differences within the studied parameters were observed. The noted certain small deviations fell within the limits of normal variations of the considered indicators and, thus, had no biological or toxicological value.
The effect of growing maize with undersown crops on the content of mycotoxins in maize biomass was studied. Small plot experiments were conducted in 2019 on two sites with different soil and climatic conditions: Žabčice and Troubsko. Three treatments of intermediate crops (Italian ryegrass; Fodder vetch and a mixture of both) were undersown into the space between the rows of maize. The maize was harvested at a dry matter content of 35% at the Troubsko experimental site and 43% at the Žabčice experimental site. After the harvest of maize, samples of green biomass (shreddings) were dried at 60°C and then analyzed for the content of mycotoxins such as deoxynivalenol (DON), aflatoxin (AF,L), and fumonisin (FUM). An average yield of maize shreddings ranged from 16.50 to 21.57 t/ha of dry matter within the individual treatment. The contents of mycotoxins from the sites differed in their statistical significance, and both experimental sites showed the lowest concentrations of AFL in maize shreddings while average concentrations of FUM and DON were always the highest. In most observations, treatments with the undersown crops reached the same values as the control treatment. Only in one treatment (mixture of Italian rye grass and Fodder vetch), an increase in the AFL content (by 0.3 µg/kg) was detected. Based on the performed analyses, it is possible to state that no adverse influence of undersown crops on the occurrence of mycotoxins in maize shreddings was recorded using the chosen methodology of cultivation. Exceeded limit values for the content of mycotoxins in feeds according to 2006/576/ were not recorded.
The aim of this work was to identify and quantify the relationships between dry matter content and key energy nutrients (water-soluble carbohydrates and starch) in silage maize hybrids (Zea mays L.) during vegetation development in the foothills of Slovakia. This work summarises the results of 9 experiments carried out at three locations (2016 to 2021: 140 different hybrids; 1,320 measured samples). Dry matter content was determined by a combination of gravimetric determination and measurement of water content in dried samples using the NIRS method (prediction model R2 0.987). We also determined the content of water soluble carbohydrates (prediction model R2 0.994) and starch (prediction model R2 0.978) in dry matter using the NIRS method. Using 3rd and 4th degree polynomial models, we evaluated the contents of these nutrients at a dry matter content level of 300 g.kg-1, which we can consider as a basic starting point for reaching maize silage maturity. Across individual years, and across groups of hybrids grouped according to silage maturity determined by dry matter content, water soluble sugar content reached 140 g.kg-1 of dry matter and starch content reached 280 g.kg-1 of dry matter at a dry matter content of 300 g.kg-1. We found the lowest variation coefficient (7.53%) when evaluating the relationship between dry matter and the sum of water-soluble carbohydrates together with starch. We suggest using this parameter as a second criterion for determining silage maturity, and also for classifying hybrids in silage maturity groups with rounding to a ten-day period.
1,2-propanediol (propanediol) represents an important glucoplastic substance for the nutritional requirements of highly productive cows, and its natural synthesis during silage fermentation is of great importance. The aim of this paper was to monitor the fermentation activity of two combinations of homo- and hetero-fermentative lactic acid bacteria (LAB) in two preparations (preparation 1: L. buchneri, L. rhamnosus, L. plantarum; preparation 2: L. buchneri, L. rhamnosus, L. diolivorans). Propanediol production was monitored in maize silages at the following levels: cutting technology, hybrid, vegetation development, type of silage fermentation and length of fermentation time. From the point of view of silage alternatives, preparation 1 fulfilled the declared properties and had a demonstrably positive influence on the production of propanediol in maize silages from whole plants (average content of 4.93 g.kg-1). Propanediol production in the silage alternative with preparation 2 (average content of 1.33 g.kg-1) was higher when compared to the negative control (average content of 0.11 g.kg-1). The production of propanediol decreased with increasing dry matter content, which is directly related to the advancing vegetative development of plants. The type of silage fermentation and vegetation development had a statistically demonstrable effect on the level of propanediol production in maize silages. A statistically significant difference at the hybrid level was only identified with one hybrid. No statistically significant differences were found at the level of cutting technology and length of fermentation time. The fermentation activity of the Lactobacillus buchneri strain used depended on its combination with other homofermentative and/or heterofermentative LAB strains.
Field trials were conducted in the early cropping seasons of 2014, 2015 and 2016 at Federal University of Agriculture, Abeokuta (7° 20‘ N, 3° 23‘ E) Nigeria to evaluate intercropped cucumber and jackbean as supplements to pre-emergence herbicides on growth and yield of maize. The treatments were arranged in a split – plot arrangement fitted into Randomized Complete Block Design (RCBD) with three replications. The six main plot treatments consisted of maize at 100 × 37.5 cm intercropped with cucumber, maize at 100 × 37.5 cm intercropped with jackbean, maize at 75 × 50 cm intercropped with cucumber, maize at 75 × 50 cm intercropped with jackbean, sole maize at 100 × 37.5 cm, sole maize at 75 × 50 cm, completed the treatments as controls. Eight treatments were assigned to the sub – plot. Data collected were subjected to Analysis of Variance while significant means were separated using Duncan`s Multiple Range Test (p <0.05). Sole maize at 75 × 50 cm and the intercrop with jackbean consistently produced significantly higher (p <0.05) shoot dry matter, compared to other cropping patterns while maize intercropped with cucumber depressed the aforementioned parameter. Propaben at 1.6 kg a.i·ha-1 followed by (fb) supplementary hoe weeding (SHW) resulted in higher stand count, vigour score, shoot dry matter and maize cob yield relative to other weed control methods. Therefore, maize should be planted at 75 × 50 cm with or without jackbean and sprayed with Propaben at 1.6 kg a.i·ha-1 fb SHW as this gave the maximum values.
The effect of different fertilizers (digestate, Ureastabil) and their combinations with strip-till/no-till technology on yield and selected quality parameters of the maize hybrid KWS Kampinos (FAO 200) in a dual cropping system (winter rye - maize) was assessed in two-year field trials (harvest 2021-2022). A significant influence of year was found in most monitored parameters. A total of 6 variants were compared, 2 variants with no-till and 4 variants with strip-till. After using the strip-till method, the silage maize yield increased significantly (11.8 – 15.4 t DM/ha) compared to the no-till method, either on the unfertilized variant (8.3 t DM/ha) or after applying fertilizer Ureastabil (11.44 t DM/ha). When using the strip-till method, no significant differences in the yield of silage maize (average over 2 years) were demonstrated between these three fertilized variants: Ureastabil (14.03 t DM/ha); digestate + Ureastabil (15.4 t DM/ha), digestate (14.2 t DM/ha) at total dose of 120 kg N/ha for each variant. The dry matter content of the silage biomass was insignificant between all the variants (32.0 – 34.1%). Differences in quality parameters of silage under the influence of fertilization variants were minimal (crude protein, starch, ash, ADF) or none (NDF). From the results obtained, it can be concluded that maize can be successfully grown and fertilized with strip-till soil conservation technology.
Despite the critical role played by roots for the acquisition of water and nutrients from the soil, especially in stressful conditions; root characteristics are rarely utilized as selection criteria to improve nutrient uptake efficiency in maize breeding programs. In this study, seedlings of twelve Algerian maize populations with two commercial maize hybrids were evaluated to: (i) study the phenotypic variation of nine root traits under low nitrogen (LN) and high nitrogen (HN) levels and (ii) determine root traits accounting for most of the phenotypic variation among the evaluated populations. High phenotypic diversity was observed for all studied traits under both N levels. Under LN conditions, seedlings adapt by rapidly increasing their root systems to exploit nitrogen (N) resources, resulting in significant increases in primary root length (PRL), seminal root length (SRL), seminal root number (SRN), and total root length (TRL). Conversely, shoot dry weight (SDW) decreased. The first two principal components explained 73.46 and 74.82% of the total variation among the maize populations under LN and HN conditions, respectively. Thus, root dry weight (RDW) and TRL were major contributors of the phenotypic variation. In addition, RDW and TRL were significantly correlated with most of the other traits under both N levels. Therefore, during the seedling stage, RDW and TRL can be used as favorable selection criteria. This diversity gives opportunities for enhancing N use efficiency, potentially leading to reducing N fertilizer requirements. As a result, this can contribute to reduce input costs and maintaining environmental quality.
Protein ratio and amino acid composition vary considerably in maize according to the level of opacity. In this study, the total protein content, amounts of some essential amino acids (lysine and tryptophan) and quantitative and qualitative variation of protein fractions in maize seed samples separated according to different opacity levels were investigated. In the study, 6 local maize landraces with opaque kernel structure and 3 standards were used. The field trial was carried out in 2021 according to the complete blocks trial design with 3 replications. Samples of local maize landraces from the field experiment were separated on a light table according to 5 different opacity levels (0%, 25%, 50%, 75% and 100%). Protein content, lysine and tryptophan contents, albumin + globulin, glutelin and zein contents were analyzed in the samples separated by their opacity. In addition, protein fractions were subjected to SDS-PAGE analysis and the changes in protein bands according to opacity level were examined. Differences between genotypes, opacity levels and their interaction were illustrated with box plot and PCA-Biplots. Two-way dendrograms were created for qualitative discrimination. The results of the study showed that there were significant changes in protein amount and quality according to the opacity levels of the genotypes. The protein contents of the genotypes were 5.95–10.22%, lysine contents 0.33–0.46%, tryptophan contents 0.032–0.083%, albumin + glubulin contents 1.92–2.72%, glutelin contents 0.64–1.08%, and zein contents 1.59–1.99%. There was a significant difference in presence or absence according to the opacity levels in the protein fractions.
This research investigates the effects of industrial fertilisers and the incorporation of plant biomass, including catch crops, on the yield and yield components of maize (Zea mays L.) within a crop rotation system. The field experiment was conducted from 2017 to 2019 at the Slovak University of Agriculture in Nitra (48°19ˊ N, 18°09ˊ E). The study period was characterized by warm to exceptionally warm and predominantly dry conditions, except for 2019, which aligned more closely with the long-term average. The experimental treatments, combining the ploughing of above-ground biomass and the application of industrial fertilisers, resulted in the highest yield component values. Notably, the number of grains per cob reached 36, and the number of grains per hectare was 27.49 million. Total seed weight (TSW) was also significantly influenced by fertilisation, with the highest TSW values ranging from 220.1 g to 225.9 g, compared to the unfertilised treatment values of 194.4 g to 207.3 g. The highest maize grain yields, ranging from 5.76 t ha⁻¹ to 6.2 t ha⁻¹, and above-ground biomass production, ranging from 12.45 t ha⁻¹ to 12.60 t ha⁻¹, were achieved in treatments incorporating both industrial fertilisers and the ploughing of biomass from forecrops or catch crops. These results underscore the importance of fertilisation, organic matter incorporation, and a balanced combination of adaptive measures in enhancing maize productivity.
In a two-year pot experiment carried out in a vegetation cage located on the premises of the Slovak University of Agriculture in Nitra, the effect of vermicompost (Vc) used at a dose 8.3 to 16.6 times higher than permitted by the Nitrates Directive on young maize plants on their phytomass was investigated. The results showed that excessive amounts of vermicompost did not have a depressing effect on the growth of young maize plants. On the contrary, they increased the production of phytomass. The legislatively determined nutrient doses should take into account the type of organic fertilizer and the different abilities of plants to convert nutrients into phytomass formation.