Kinetics of Acrylamide-Induced Changes in Ionome Composition in Schizosaccharomyces pombe

In stressful situations, maintaining ionome homeostasis in the intracellular space is a key prerequisite for cell survival. This study investigates the role of ionome alteration due to acrylamide-induced oxidative stress in Schizosaccharomyces pombe. With the use of inductively coupled plasma-optical emission spectroscopy (ICP-OES) we have evaluated changes in the intracellular concentration of eight mineral elements (calcium, potassium, sodium, magnesium, manganese, zinc, iron, copper). In this study, the deleterious effect of acrylamide (1 and 10 mM) on the uptake and utilization of mineral elements and the disruption of ionome homeostasis has been revealed. We confirmed that the toxicity of acrylamide (mainly 10 mM concentration) for cells and their biological activity is associated with oxidative stress due to enhanced accumulation of reactive oxygen species (ROS) and increased formation of malondialdehyde (MDA). The addition of acrylamide into cultivation media in a final concentration of 10 mM led to 72.3% increase in total ROS production. At the same time, this resulted in statistically significant changes in the ionome, accompanied by an increase in the concentrations of monovalent ions K+ and Na+, divalent ions Ca2+, Mg2+, Cu2+, and trivalent Fe3+. These findings shed light on the complexity of the acrylamide-induced metabolic disorders that lead to the imbalance of ion homeostasis.